Analytical chemistry

Enzymically amplified voltammetric sensor for microliter sample volumes of salicylate

…, MJ Joseph, BW Allen, LA Coury Jr

Index: Moore, Thea J.; Joseph, Melissa J.; Allen, Barry W.; Coury Jr., Louis A. Analytical Chemistry, 1995 , vol. 67, # 11 p. 1896 - 1902

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Citation Number: 45

Abstract

A new voltammefric sensing strategy for salicylate employ-ing two enzymes and applicable to microliter sample volumes is demonstrated. The method involves the use of the enzyme salicylate hydrowlase to convert salicylate to catechol, which is oxidized at a carbon electrode. The product of this oxidation reaction, o-quinone, is then reduced by a second enzyme, glucose oxidase, to regenerate catechol. Reoxidation of catechol results in a ...