Quantitative High-Performance Liquid Chromatography− Electrospray Ionization Tandem Mass Spectrometry Analysis of Bis-N 7-Guanine DNA− DNA Cross-Links in …

B Malayappan, LA Johnson, B Nie, D Panchal…

Index: Malayappan, Bhaskar; Johnson, L'aurelle; Nie, Bei; Panchal, Dolly; Matter, Brock; Jacobson, Pamala; Tretyakova, Natalia Analytical Chemistry, 2010 , vol. 82, # 9 p. 3650 - 3658

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Citation Number: 24

Abstract

Cyclophosphamide (CPA) is a DNA alkylating agent widely used in cancer chemotherapy. CPA undergoes metabolic activation to phosphoramide mustard and nornitrogen mustard (NOR) which alkylate the N-7 position of guanine in DNA to produce N-[2-(N 7-guaninyl) ethyl]-N-[2-hydroxyethyl]-amine (G-NOR-OH) monoadducts and N, N-bis [2-(N 7-guaninyl) ethyl] amine cross-links (G-NOR-G). G-NOR-G cross-links are strongly cytotoxic and are ...