Abstract Short, dye-labeled oligonucleotides have been used as primers in template- controlled polymerization reactions of RNA. The synthesis of appropriate acridine derivatives and their attachments to nucleic acids is described. In the nonenzymatic oligomerization of 2-methyl-1H-imidazole-activated guanosine 5′-monophosphate, two observations deserve special notice:(1) reaction rates are almost unchanged by variations ...
