Photochemically amplified detection of molecular recognition events: an ultra-sensitive fluorescence turn-off binding assay

…, GA Metzel, AG Kutateladze

Index: Gustafson, Tiffany P.; Metzel, Greg A.; Kutateladze, Andrei G. Organic and Biomolecular Chemistry, 2011 , vol. 9, # 13 p. 4752 - 4755

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Citation Number: 12

Abstract

Amplified fluorescence quenching methodology based on massive autocatalytic photo- unmasking of a dual function sensitizer–quencher is developed and adopted for photoassisted ultra-sensitive detection of molecular recognition events. The resulting binding assay, based on a molecular recognition-triggered photo-amplified cascade with concomitant decrease of fluorescence is validated with the biotin–avidin pair, achieving ...