We report two-photon (TP) pH probes (AH1, AH2) and a TP lysotracker (AL1, Scheme 1) derived from 2-acetyl-6-(dimethylamino) naphthalene (acedan). These probes can visualize the acidic vesicles in live cells and tissues. Lysosomes and lysosome-related organelles constitute a system of acidic compartments (pH 4.0–5.0) that contain a large number of enzymes and secretory proteins exhibiting a variety of functions.[1, 2] To determine their ...
