Organic & biomolecular chemistry

The synthesis of phosphopeptides via the Bpoc-based approach

TJ Attard, EC Reynolds, JW Perich

Index: Attard, Troy J.; Reynolds, Eric C.; Perich, John W. Organic and Biomolecular Chemistry, 2007 , vol. 5, # 4 p. 664 - 670

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Citation Number: 5

Abstract

The 2-(p-biphenylyl)-2-propyloxycarbonyl (Bpoc) group was examined as an Nα-protecting group in the stepwise assembly of the MAP Kinase ERK2 [178–188; Thr (P) 183, Tyr (P) 185] peptide. The mild acid deprotection of the Bpoc group permitted (i) incorporation of a fully protected phosphothreonyl derivative and (ii) a TFA-based final cleavage step. The first five C-terminal residues (184–188) were incorporated in the Fmoc mode of peptide synthesis, ...