Comparison of two vectors for functional expression of a bacterial cytochrome P450 gene in Escherichia coli using CYP153 genes

N Fujita, F Sumisa, K Shindo, H Kabumoto…

Index: Fujita, Naoya; Sumisa, Futoshi; Shindo, Kazutoshi; Kabumoto, Hiroki; Arisawa, Akira; Ikenaga, Hiroshi; Misawa, Norihiko Bioscience, Biotechnology and Biochemistry, 2009 , vol. 73, # 8 p. 1825 - 1830

Full Text: HTML

Citation Number: 28

Abstract

Two vectors, pT7NScamAB and pRED, have been used for the functional expression of bacterial class I cytochrome P450 (P450) genes in Escherichia coli, which utilize putidaredoxin reductase (CamA) and putidaredoxin (CamB), and the reductase domain of a self-sufficient P450RhF respectively, for electron transfer from NAD (P) H to a P450 protein. We here compared the efficiency of bioconversion with the two vectors towards n-octane, ...