A new glycoside, hypoxoside, was isolated from rhizome of Hypoxis obtusa, a plant used in Mozambique in native medicine for the treatment of urinary diseases. The structure 1 of a diglycoside of 1-(3′, 4′-dihydroxyphenyl)-5-(3 ″, 4 ″-dihydroxyphenyl)-1-penten-4-yne was attributed to hypoxoside on the basis of physico-chemical determinations and chemical behaviour.