5'-S-(2-Sulphoethyl)-5'-deoxy-5'-thioadenosine (S-adenosylcoenzyme M), 5, was prepared from adenosine by reacting its 2', 3'-isopropylidine tosylate with the disodium salt of coenzyme M (NaSCH2CH2SO3Na) followed by removal of the protecting group. Methylation of 5 to give methylsulphonium (±) S-adenosylcoenzyme M, 6, was achieved with methyl iodide in acetic acid. Neither 5 nor 6 stimulated methane production from cell free ...
