Optimized assays for human UDP-glucuronosyltransferase (UGT) activities: altered alamethicin concentration and utility to screen for UGT inhibitors

…, K Bourcier, G Giddens, K Lapham, A Negahban…

Index: Walsky, Robert L.; Bauman, Jonathan N.; Bourcier, Karine; Giddens, Georgina; Lapham, Kimberly; Negahban, Andre; Ryder, Tim F.; Obach, R. Scott; Hyland, Ruth; Goosen, Theunis C. Drug Metabolism and Disposition, 2012 , vol. 40, # 5 p. 1051 - 1065

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Citation Number: 63

Abstract

Abstract The measurement of the effect of new chemical entities on human UDP- glucuronosyltransferase (UGT) marker activities using in vitro experimentation represents an important experimental approach in drug development to guide clinical drug-interaction study designs or support claims that no in vivo interaction will occur. Selective high- performance liquid chromatography-tandem mass spectrometry functional assays of ...