Enzymatic synthesis of phenylpyruvic acid labeled with deuterium, tritium, and carbon??14

K Skowera, M Kańska

Index: Skowera, Katarzyna; Kanska, Marianna Journal of Labelled Compounds and Radiopharmaceuticals, 2008 , vol. 51, # 8 p. 321 - 324

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Citation Number: 3

Abstract

Abstract The synthesis of isotopomers of phenylpyruvic acid, PPA, selectively labeled with hydrogen isotopes in the 3-position of the side-chain is reported. Three deuterium or tritium labeled isotopomers of L-phenylalanine, L-Phe, ie [(3S)-2 H]-L-,[(3S)-3 H]-L-, and doubly labeled [(3S)-2 H/3 H]-L-Phe were synthesized using the enzyme phenylalanine ammonia lyase (EC 4.3. 1.5). In the second step these isotopomers of L-Phe were converted into [( ...