Enantioselective synthesis of non-natural amino acids using phenylalanine dehydrogenases modified by site-directed mutagenesis

F Paradisi, E Moynihan, AR Maguire…

Index: Busca, Patricia; Paradisi, Francesca; Moynihan, Eamonn; Maguire, Anita R.; Engel, Paul C. Organic and Biomolecular Chemistry, 2004 , vol. 2, # 18 p. 2684 - 2691

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Citation Number: 23

Abstract

The substrate scope of three mutants of phenylalanine dehydrogenase as biocatalysts for the transformation of a series of 2-oxo acids, structurally related to phenylpyruvic acid, to the analogous α-amino acids, non-natural analogues of phenylalanine, has been investigated. The mutant enzymes are more tolerant than the wild type enzyme of the non-natural substrates, especially those with substituents at the 4-position on the phenyl ring. ...