Novel chromone derivatives with a benzopyran-4-one scaffold have been prepared by the one-pot cyclization reaction. The in vitro inhibitory activity of these new compounds towards HIV-1 protease have been evaluated using stop time HPLC method as the preliminary screening. The most potent compound, 7, 8-dihydroxy-2-(3′-trifluoromethyl phenyl)-3-(3 ″- trifluoromethylbenzoyl) chromone (32), showed IC50= 0.34 μM. The molecular docking ...