During our studies on denaturation with synthetic agents, we observed that strong binding to cyt c is not a prerequisite for denaturing activity. Indeed, the stronger the binding to the native protein, the weaker any denaturing effect will be. It is likely that selective binding to the denatured state, or any of a series of non-native states, over the native state is the determining factor in the ability of a porphyrin to denature a protein. Charged peripheral groups that ...
