The substrate specificity at the active site of recombinant human synovial fluid phospholipase Az (hs-PLA2) was investigated by the preparation of a series of short-chain phospholipid analogs and measurement of their enzymatic hydrolysis at concentrations well below the critical micelle concentration. Substrates used in the study included 1, 2- dihexanoylglycerophospholipids, 1, 2-bis (alkanoylthio) glycerophospholipids, and lO- ...
