A stereoselective and highly practical synthesis of cytosolic phospholipase A2 substrate, 2-S-arachidonoyl-1-O-hexadecyl-sn-2-thioglycero-3-O-phosphocholine

M Fuji, F Watanabe, Y Fujii, H Hashizume…

Index: Fuji, Masahiro; Watanabe, Fumihiko; Fujii, Yasuhiko; Hashizume, Hiroshi; Okuno, Takayuki; Shirahase, Kazuhiro; Teshirogi, Isao; Ohtani, Mitsuaki Journal of Organic Chemistry, 1997 , vol. 62, # 20 p. 6804 - 6809

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Citation Number: 14

Abstract

The substrate 1 of cytosolic phospholipase A2 (cPLA2) is an ether-type thiophospholipid with arachidonic acid at the C-2 position and is required for the chromogenic assay for reliable and convenient high throughput screening. The original method of synthesis of 1 has significant problems, resulting in extremely low overall yield and purity. We developed a novel and highly practical method of preparing sufficient quantities of pure 1 for assay. Our ...