The gene for one of the glycosyltransferases from Populus deltoids, PGT-3, was cloned and was expressed as a glutathione S-transferase fusion protein in Escherichia coli. Various flavonoids were used as potential substrates of the purified recombinant PGT-3. Flavones having two adjacent hydroxyl groups were served as substrate. The regioselectivity of PGT-3 depends on the hydroxyl groups of the substrate. Flavones having two adjacent hydroxyl ...
