Biochemistry

Unexpected formation of an epoxide-derived multisubstrate adduct inhibitor on the active site of GAR transformylase

SE Greasley, TH Marsilje, H Cai, S Baker…

Index: Bigham, Eric C.; Mallory, W. Revill; Hodson, Steven J.; Duch, David S.; Ferone, Robert; Smith, Gary K. Heterocycles, 1993 , vol. 35, # 2 p. 1289 - 1307

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Citation Number: 33

Abstract

... cofactor and substrate, β-GAR, have demonstrated superb potency against GAR Tfase, with K i 's in the ... formyl-TDAF) (4) (Figure 1), although not a particularly potent inhibitor toward GAR Tfase (K i of ... Purified enzyme was stored at 4 °C, at a concentration of 2.6 mg/mL in 50 mM ...