On the role of copper and iron in DNA cleavage by ochratoxin A. Structure-activity relationships in metal binding and copper-mediated DNA cleavage

…, IG Gillman, RA Manderville

Index: Ardus, Jason A.; Gillman, Ivan G.; Manderville, Richard A. Canadian Journal of Chemistry, 1998 , vol. 76, # 6 p. 907 - 918

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Citation Number: 16

Abstract

Ochratoxin A (OTA, 1: X= Cl) is a fungal carcinogen that facilitates single-strand DNA cleavage and DNA adduction when metabolically activated. To determine if redox-active transition metals induce OTA-mediated DNA damage, we have examined the toxin's ability to bind Cu (II) and Fe (III) in aqueous media and facilitate DNA cleavage in their presence using agarose gel electrophoresis and supercoiled plasmid DNA. Using fluorescence ...