Squaric monoamide monoester as a new class of reactive immunization hapten for catalytic antibodies

…, N Yamamoto, DI Ruiz, DS Kubitz, KD Janda

Index: Xu, Yang; Yamamoto, Noboru; Ruiz, Diana I.; Kubitz, Diane S.; Janda, Kim D. Bioorganic and Medicinal Chemistry Letters, 2005 , vol. 15, # 19 p. 4304 - 4307

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Citation Number: 13

Abstract

A squaric monoester monoamide motif was employed as an effective reactive immunogen for the discovery of monoclonal antibodies with reactive residue (s) in their combining sites. Two antibodies, 2D4 and 3C8, were uncovered that enhance paraoxon hydrolysis over background. Kinetic analysis of these antibodies was performed and interestingly both undergo a single turnover event due to covalent modification within the antibody ...