Synthesis and utility of a DNA phosphorylating agent based on 2-(Triphenylsilyl) ethanol

JE Celebuski, C Chan, RA Jones

Index: Celebuski, Joseph E.; Chan, Clifford; Jones, Roger A. Journal of Organic Chemistry, 1992 , vol. 57, # 20 p. 5535 - 5538

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Citation Number: 16

Abstract

The enzyme DNA ligase catalyzes the template-driven condensation of the 3'-hydroxyl group of one DNA strand with the 5'-monophosphata group of a second DNA strand. As part of a study of DNA ligase, we required a method for 5'-phosphorylation of an oligonucleotide during phosphoramidite-based, automated DNA synthesis.'Fur-thermore, this phosphorylated oligonucleotide needed to be separable, by reversed-phase HPLC, from ...