Enantioselective hydrolysis of some 2-aryloxyalkanoic acid methyl esters and isosteric analogues using a penicillin G acylase-based HPLC monolithic silica column

G Massolini, E Calleri, A Lavecchia, F Loiodice…

Index: Massolini, Gabriella; Calleri, Enrica; Lavecchia, Antonio; Loiodice, Fulvio; Lubda, Dieter; Temporini, Caterina; Fracchiolla, Giuseppe; Tortorella, Paolo; Novellino, Ettore; Caccialanza, Gabriele Analytical Chemistry, 2003 , vol. 75, # 3 p. 535 - 542

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Citation Number: 52

Abstract

A technique based on liquid chromatography has been developed to facilitate studies of enantioselectivity in penicillin G acylase (PGA)-catalyzed hydrolysis of some 2- aryloxyalkanoic acid methyl esters and isosteric analogues. PGA was covalently immobilized on an aminopropyl monolithic silica support to create an immobilized HPLC- enzyme reactor. Two sets of experimental data were drawn to calculate the ...