Chemistry–A European Journal

Discovery of new biocatalysts for the glycosylation of terpenoid scaffolds

L Caputi, EK Lim, DJ Bowles

Index: Caputi, Lorenzo; Lim, Eng-Kiat; Bowles, Dianna J. Chemistry - A European Journal, 2008 , vol. 14, # 22 p. 6656 - 6662

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Citation Number: 42

Abstract

Abstract The synthesis of terpenoid glycosides typically uses a chemical strategy since few biocatalysts have been identified that recognise these scaffolds. In this study, a platform of 107 recombinant glycosyltransferases (GTs), comprising the multigene family of small molecule GTs of Arabidopsis thaliana have been screened against a range of model terpenoid acceptors to identify those enzymes with high activity. Twenty-seven GTs are ...