The 4-pentenoyl group and a number of derivatives have been studied as protecting groups for N α of the aminoacyl moiety in misacylated tRNAs. The unsubstituted 4-pentenoyl group itself was found to function as efficiently as any of the derivatives studied. Four different N-(4- pentenoyl) aminoacyl-tRNACUAs were prepared and shown to undergo deprotection readily upon admixture of aqueous iodine; the derived misacylated tRNAs all functioned ...
