Stereochemical and isotopic labeling studies of 2-oxo-hept-4-ene-1, 7-dioate hydratase: evidence for an enzyme-catalyzed ketonization step in the hydration reaction

…, WH Johnson, CP Whitman

Index: Burks, Elizabeth A.; Johnson Jr., William H.; Whitman, Christian P. Journal of the American Chemical Society, 1998 , vol. 120, # 31 p. 7665 - 7675

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Citation Number: 18

Abstract

2-Oxo-hept-4-ene-1, 7-dioate hydratase from Escherichia coli C converts 2-oxo-hept-4-ene- 1, 7-dioate to 2-oxo-4-hydroxy-hepta-1, 7-dioate by the addition of water using magnesium as a cofactor. The enzyme is one of a set of inducible enzymes, known collectively as the homoprotocatechuate meta-fission pathway. The entire pathway enables the organism to utilize aromatic amino acids as its sole sources of carbon and energy. Expression and ...