Biosynthesis of branched-chain fatty acid in Bacilli: FabD (malonyl-CoA: ACP transacylase) is not essential for in vitro biosynthesis of branched-chain fatty acids

H Oku, N Futamori, K Masuda…

Index: Bioscience, biotechnology, and biochemistry, , vol. 67, # 10 p. 2106 - 2114

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Abstract

... Division of Molecular Biotechnology, Center of Molecular Bioscience, University of the Ryukyus ... The expressed His-tag protein was puriˆed to homogeneity by Ni2＋ chelate a‹nity chromatography, and thereafter desalted with a PD-10 column (Amersham-Pharmacia Biotech). ...