Conversion of a carboxylesterase into a triacylglycerol lipase by a random mutation

…, J Polaina, N López??Cortés, M Alcalde…

Index: Reyes-Duarte, Dolores; Polaina, Julio; Lopez-Cortes, Nieves; Alcalde, Miguel; Plou, Francisco J.; Elborough, Kieran; Ballesteros, Antonio; Timmis, Kenneth N.; Golyshin, Peter N.; Ferrer, Manuel Angewandte Chemie - International Edition, 2005 , vol. 44, # 46 p. 7553 - 7557

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Citation Number: 37

Abstract

Lipases and esterases are enzymes of increasing importance for classical and new industrial applications.[1, 2] The most significant properties of these enzymes are that they are very stable and active, especially in organic solvents, and they possess regio-and stereospecificity.[2] Esterases (also called carboxylesterases, EC 3.1. 1.1) preferentially hydrolyze watersoluble “simple” esters and usually only triglycerides bearing fatty acids ...