Application of Brønsted-type LFER in the study of the phospholipase C mechanism

…, AV Kravchuk, MD Tsai, KS Bruzik

Index: Mihai, Cornelia; Kravchuk, Alexander V.; Tsai, Ming-Daw; Bruzik, Karol S. Journal of the American Chemical Society, 2003 , vol. 125, # 11 p. 3236 - 3242

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Citation Number: 20

Abstract

Phosphatidylinositol-specific phospholipase C cleaves the phosphodiester bond of phosphatidylinositol to form inositol 1, 2-cyclic phosphate and diacylglycerol. This enzyme also accepts a variety of alkyl and aryl inositol phosphates as substrates, making it a suitable model enzyme for studying mechanism of phosphoryl transfer by probing the linear free- energy relationship (LFER). In this work, we conducted a study of Brønsted-type ...