Cytochrome P450BM3 and its F87V mutant were exploited for a convenient, laboratory scale (1 mmol) preparation of 14 (S), 15 (R)-epoxyeicosatrienoic acid [14 (S), 15 (R)-EET] from arachidonic acid and (+)-leukotoxin B [(+)-12 (S), 13 (R)-vernolic acid] from linoleic acid, respectively. Their enantiomers were accessed via a four-step chemical inversion.