Abstract Two types of chromogenic substrates for papain were prepared, namely p- nitroanilides of N-acetyltripeptides of general formula Ac-Leu-Leu-A-NAn, where A= Gly, Ala, Leu, and p-nitroanilides of 3-carboxypropionyldi-and tripeptides of general formula Suc-B- Cys (Bzl)-NAn, where B= Gly, Gly-Gly, Ala, Ala-Ala, Leu, Leu-Leu, Gly-Leu, Leu-Ala and Ala- Met. The values of K m, k cat and C (k cat/K m) for these substrates were determined. Suc- ...
