Synthesis of guanosine and deoxyguanosine phosphoramidites with cross-linkable thioalkyl tethers for direct incorporation into RNA and DNA

…, G Wang, BL Gaffney, RA Jones

Index: Hou, Xiaorong; Wang, Gang; Gaffney, Barbara L.; Jones, Roger A. Nucleosides, Nucleotides and Nucleic Acids, 2009 , vol. 28, # 11-12 p. 1076 - 1094

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Citation Number: 5

Abstract

We describe the synthesis of protected phosphoramidites of deoxyriboguanosine and guanosine derivatives containing a thiopropyl tether at the guanine N2 (7a, b) for site- specific crosslinking from the minor groove of either DNA or RNA to a thiol of a protein or another nucleic acid. The thiol is initially protected as a tert-butyl disulfide that is stable during oligonucleotide synthesis. While the completed oligonucleotide is still attached to ...