Abstract A detailed analysis of Bupleurum fruticosum oil was carried out by combination of GC (RI), GC/MS, and 13 C-NMR analyses. After fractionation by column chromatography, 34 components accounting for 97.8% of the oils were identified. The main component was β- phellandrene (67.7%), followed by sabinene (9.3%), and limonene (5.6%). The evolution of the chemical composition according to the stages of development of the plant was ...