Isotope and affinity tags in photoreactive substance P analogues to identify the covalent linkage within the NK-1 receptor by MALDI-TOF analysis

E Sachon, O Tasseau, S Lavielle, S Sagan…

Index: Sachon, Emmanuelle; Tasseau, Olivier; Lavielle, Solange; Sagan, Sandrine; Bolbach, Gerard Analytical Chemistry, 2003 , vol. 75, # 23 p. 6536 - 6543

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Citation Number: 14

Abstract

Photoreactive analogues of substance P (biotin sulfone-spacer (amino pentanoic or Gly3)- Arg-Pro-Lys-Pro-(p Bzl) Phe-Gln-Phe-Phe-Gly-Leu-Met (O2) NH2) with or without isotope (deuterium) labeling have been synthesized. Deuteriums were present on (d)-biotin or epibiotin sulfone (D3), on the Gly3 spacer linker (D6), or on the Gly in position 9 of SP (D2). Therefore, peptide analogues could be either unlabeled or tri-, penta-, or hexadeuterated. ...