A selective method was developed for the purification of synthetic peptides. It is based on the addition of the 9-(2-sulfo) fluorenylmethyloxycarbonyl (Sulfmoc) group to the free amine of the growing peptide chains at the end of a solid-phase synthesis. After cleavage from the support, the strongly acidic Sulfmoc-peptides are separated chromatographically from the nongrowing, terminated peptides. Finally, the Sulfmoc group is removed by mild base and ...
