... Biotech. ... activity were prepared by growing them in a medium containing 0.5% glycerol, 0.5% yeast extract, 0.5% polypepton, 3.0 vol% soybean protein ... mg pyridoxal 5'-phosphate, 0.25mmol ofNH40H-NH4CI buffer, pH 9.0, and 200mg of lyophilized cells in a total volume of lOml ...