Synthesis and application of a photoaffinity analog of dehydroepiandrosterone (DHEA)

…, N Perez-Hernandez, D Liu, M Iruthayanathan…

Index: Olivo, Horacio F.; Perez-Hernandez, Nury; Liu, Dongmin; Iruthayanathan, Mary; O'Leary, Brianne; Homan, Laurie L.; Dillon, Joseph S. Bioorganic and Medicinal Chemistry Letters, 2010 , vol. 20, # 3 p. 1153 - 1155

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Citation Number: 7

Abstract

We have synthesized an analog of dehydroepiandrosterone (DHEA, 1) containing both a benzophenone (BP) and a biotin (Bt) group (DHEA–BP–Bt, 8). Compound 8 was prepared by functionalization on C-17 of 1. Biocytin was reacted with 4-benzoylbenzoic acid and the product was condensed with 1 containing a diamine–hexane linker. We detected specific protein bands of approximately 55, 80, and 150kDa by SDS–PAGE analysis of vascular ...