D, O) at room temperature, and the solution was quickly frozen in an acetone/dry ice bath. To the almost frozen solution, was carefully added 66.6 FL of 36 N H2S04. The pH-2 solution was added to a tris-base buffer solution. Up to this point everything was carried out within a minute. Some inorganic precipitate was filtered through a nitric acid and EDTA solution treated disposable pipet plugged with EDTA-treated cotton and Chelex-100 (Bio-Rad) ion ...
