Abstract Nitrile reductase QueF catalyzes the reduction of 2-amino-5-cyanopyrrolo [2, 3-d] pyrimidin-4-one (preQ 0) to 2-amino-5-aminomethylpyrrolo [2, 3-d] pyrimidin-4-one (preQ 1) in the biosynthetic pathway of the hypermodified nucleoside queuosine. It is the only enzyme known to catalyze a reduction of a nitrile to its corresponding primary amine and could therefore expand the toolbox of biocatalytic reactions of nitriles. To evaluate this ...
![6-bromo-4-chloro-5-cyanopyrrolo[2,3-d]pyrimidin-4-one Structure](https://image.chemsrc.com/caspic/324/24391-39-7.png)
![7H-pyrrolo[2,3-d]pyrimidine-5-carbonitrile Structure](https://image.chemsrc.com/caspic/274/454685-88-2.png)