PNA-DNA chimeras containing 5-alkynyl-pyrimidine PNA units. Synthesis, binding properties, and enzymatic stability

…, G Sági, Z Tegyey†, F Kraicsovits

Index: Bajor, Zoltan; Sagi, Gyula; Tegyey, Zsuzsanna; Kraicsovits, Ferenc Nucleosides, Nucleotides and Nucleic Acids, 2003 , vol. 22, # 10 p. 1963 - 1983

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Citation Number: 13

Abstract

Abstract Three chimeric dimer synthons (oeg_tNHT, oeg_upNHT and oeg_uhNHT) containing thymine (t), 5-(l-propynyl)-uracil (up) and 5-(1-hexyn-1-yl)-uracil (uh) PNA units with N-(2-hydroxyethyl) glycine (oeg) backbone were synthesized in solution and incorporated into T20 oligonucleotide analogues, using standard P-amidite chemistry. Insertion of dimer blocks led to destabilization of duplexes with dA20 target. The smallest ...