Bioorganic chemistry

The importance of the amide bond nearest the thiol group in enzymatic reactions of coenzyme A

J Xun, H Huang, KW Vogel, DG Drueckhammer

Index: Xun, Jin; Huang, Haidong; Vogel, Kurt W.; Drueckhammer, Dale G. Bioorganic Chemistry, 2005 , vol. 33, # 2 p. 90 - 107

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Citation Number: 4

Abstract

Analogues of coenzyme A (CoA) and of CoA thioesters have been prepared in which the amide bond nearest the thiol group has been modified. An analogue of acetyl-CoA in which this amide bond is replaced with an ester linkage was a good substrate for the enzymes carnitine acetyltransferase, chloramphenicol acetyltransferase, and citrate synthase, with Km values 2-to 8-fold higher than those of acetyl-CoA and Vmax values from 14 to> 80% ...