Bicyclomycin (1) is a commercially available antibiotic whose primary site of action in Escherichia coli is the transcription termination factor rho. Key aspects of the 1⊙ rho interaction K d, stoichiometry for 1⊙ rho binding, and whether 1 and ATP binding induce conformational changes in rho remain unknown. In this study, the design, synthesis, and characterization of a series of bicyclomycin fluorescent probes (BFP) constructed to sense ...
