Chemical primer extension: efficiently determining single nucleotides in DNA

…, A Hochgesand, U Plutowski, C Richert

Index: Plutowski, Ulrich; Richert, Clemens Angewandte Chemie - International Edition, 2005 , vol. 44, # 4 p. 621 - 625

Full Text: HTML

Citation Number: 48

Abstract

The replication and transcription of genetic information requires the stepwise extension of DNA or RNA primers in sequence-specific, templatedirected reactions (Scheme1). Template- directed primer extension also underlies PCR,[1] dideoxy sequencing,[2] and attempts to recreate life from inanimate materials.[3] Primer extension presents a formidable chemical challenge. Four different, richly functionalized monomers (the mononucleotides) have to ...