Identification of a new endogenous metabolite and the characterization of its protein interactions through an immobilization approach

J Kalisiak, SA Trauger, E Kalisiak…

Index: Kalisiak, Jarostaw; Trauger, Sunia A.; Kalisiak, Ewa; Morita, Hirotoshi; Fokin, Valery V.; et al. Journal of the American Chemical Society, 2009 , vol. 131, p. 378 - 386

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Citation Number: 31

Abstract

The emerging field of global mass-based metabolomics provides a platform for discovering unknown metabolites and their specific biochemical pathways. We report the identification of a new endogenous metabolite, N 4-(N-acetylaminopropyl) spermidine and the use of a novel proteomics based method for the investigation of its protein interaction using metabolite immobilization on agarose beads. The metabolite was isolated from the ...