Abstract Near infrared (NIR) dyes attracted increasing interests in widely potential applications, such as fluorescent probe and living organism imaging, due to their low background signals from biomolecules, low light scattering and deep penetration, and low- cost excitation light sources. A novel NIR violanthrone derivative (VA-CN) with dicyanomethylene substitution was synthesized and fully characterized by 1 H NMR, 13 C ...