An improved method for the synthesis of oligodeoxyribonucleotides containing the endogenous adduct, pyrimido [1, 2-a] purin-10 (3 H)-one (M1G), is reported. The key features of the methodology include improved synthesis of the deoxynucleoside of M1G by transribosylation with deoxycytidine catalyzed by nucleoside 2'-deoxyribosyltransferase and the use of commercially available 4-tert-butylphenoxyacetyl protecting groups for normal ...
