Synthesis of an artificial glycoconjugate polymer carrying P k-antigenic trisaccharide and its potent neutralization activity against Shiga-like toxin

…, T Kobayashi, T Takeda, H Uzawa, K Kobayashi

Index: Dohi, Hirofumi; Nishida, Yoshihiro; Mizuno, Mitsuharu; Shinkai, Masashige; Kobayashi, Takeshi; Takeda, Tae; Uzawa, Hirotaka; Kobayashi, Kazukiyo Bioorganic and Medicinal Chemistry, 1999 , vol. 7, # 9 p. 2053 - 2062

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Citation Number: 98

Abstract

Fluorescence-labeled glycoconjugate polymers carrying carbohydrate segments of a globotriaosyl ceramide (Gb3) were synthesized and subjected to biological assays using Escherichia coli O-157 strains and Shiga-like toxins (Stx-I and Stx-II). For the fluorescence labeling, a new polymerizable fluorescent monomer with a TBMB carbonyl chromophore (Ex. 325 nm, Em. 410 nm) was designed. A glycosyl monomer of the trisaccharide ...