Abstract Extracellular α-N-acetylgalactosaminidase from Aspergillus niger catalyzed glycosylation yielding a series of 2-acetamido-2-deoxy-α-D-galactobiosides using 2- acetamido-2-deoxy-D-galactopyranose as a glycosyl donor. The isomers α-D-Gal p NAc- (1→ 6)-D-Gal p NAc, α-D-Gal p NAc-(1→ 3)-D-GalpNAc and α-D-Gal p NAc-(1→ 6)-D-Gal f NAc were isolated and spectrally characterized. The purified enzyme was further used for ...