Tetrahedron

Cephalosporin biosynthesis: A branched pathway sensitive to an isotope effect

JE Baldwin, RM Adlington, NP Crouch, CJ Schofield…

Index: Baldwin; Adlington; Crouch; Schofield; Turner; Aplin Tetrahedron, 1991 , vol. 47, # 47 p. 9881 - 9900

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Citation Number: 43

Abstract

Incubation of penicillin N (3a) with partially purified deacetoxy/deacetylcephalosporin C synthase (DAOC/DAC synthase) from Cephalosporium acremonium CO 728 gave in addition to the expected products, deacetoxycephalosporin C and deacetylcephalosporin C, a third β-lactam metabolite as a 3β-hydroxy-3α-methylcepham (9a). Production of the 3β- hydroxycepham was promoted from [3-2H] penicillin N (3b) which was rationalised by the ...