Site-Directed Mutagenesis of the Sterol Methyl Transferase Active Site from Saccharomyces c erevisiae Results in Formation of Novel 24-Ethyl Sterols

WD Nes, BS McCourt, JA Marshall, J Ma…

Index: Nes, W. David; McCourt, Brian S.; Marshall, Julie A.; Ma, Jianzhong; Dennis, Allen L.; Lopez, Monica; Li, Haoxia; He, Ling Journal of Organic Chemistry, 1999 , vol. 64, # 5 p. 1535 - 1542

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Citation Number: 35

Abstract

Δ24 (28)-Sterols are end products of a mono C-methylation pathway catalyzed by the native Δ24 (25)-to Δ24 (28)-sterol methyl transferase (SMT) enzyme from Saccharomyces cerevisiae. Using a Tyr81 to Phe mutant SMT enzyme of S. cerevisiae, generated by site- directed mutagenesis of a highly conserved residue in the sterol binding site, we found that several Δ24 (25)-and Δ24 (28)-sterols, which are not substrates for the native protein, ...