Biochemistry

Protonation state of methyltetrahydrofolate in a binary complex with cobalamin-dependent methionine synthase

AE Smith, RG Matthews

Index: Hilhorst, Ellen; Iskander, Atef S.; Chen, Tjoe B. R. A.; Pandit, Upendra K. Tetrahedron, 1994 , vol. 50, # 29 p. 8863 - 8870

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Citation Number: 36

Abstract

... An equal volume of buffer was added to the front compartment of the reference cuvette. ... Rapid centrifuge filtration was used to measure a K d for CH 3 -H 4 folate binding to MetH(2−649) (34 ... A stock solution of (6R,S)-[methyl - 14 C]CH 3 -H 4 folate was dissolved in 8 mM sodium ...